NUMERICAL Fo VALUE
The actual observation obtained during the heat penetration studies at different temperature sensing location are complies in the table and the observed temperature shall be subjected to fo calculation at that particular location the lethality factor calculation is done by using the formula
F0 = dt ∑10(T-121)/z
F0 = dt ∑(sum of lethality factor)
Where,
dt = time interval between successive temperature measurements.
T =observed temperature at that particular time (as actual temperature recoded).
Z = change in the heat resistance of Geobacillus stearothermophilus
Spores as temperature is changed (10°C or mentioned in COA).
Fo VALUE FOR BIOLOGICAL INDICATORS
The biological F0 value for biological indicator strip exposed during the sterilization can be calculated as follows
F0 = D121 (log A – log B)
Where,
D121 = D Value of the biological indicator at 121°C
A =experimental biological indicator concentration or spore population.
B =desired level of sterility (SAL - 10^6)
DESIRED SPORE LOG REDUCTION
Calculate the desired reduction in spore log population by using the formula
SLR Desired = log A – log SAL desired
Where,
A = experimental population of biological indicator
SAL desired = desired level level of sterility (10-6)
ACTUAL SPORE LOG REDUCTION
Calculate the actual reduction in spore population by using the formula
SLR actual = Fo/D121
Where,
Fo = Minimum calculated Fo value
D121 = D value of Biological indicator
Appreciable and informative. I visited it the very first time. And I appreciate your hard work. You can see my site if you want to find more on Biological Indicators. Biological Indicators are test systems that contain sensible microorganisms with a portrayed insurance from a specific purifying collaboration.
ReplyDelete